About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
MetaSystems DM2019 Largest in History
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XL t(8;14) MYC/IGH DF 8cen
Translocation/Dual Fusion and Amplification Probe
- Order Number
- D-5125-100-TC
- Package Size
- 100 µl (10 Tests)
- Regulatory Status
- IVDD
IVDR Certification
MetaSystems Probes has already certified a large part of its portfolio, according to IVDR. For organizational reasons, we currently provide only the IVDD product.
Discover all IVDR-certified products
XL t(8;14) MYC/IGH DF 8cen consists of an aqua-labeled probe hybridizing to the centromere of chromosome 8, an orange-labeled probe hybridizing to the MYC gene region at 8q24.21 and a green-labeled probe hybridizing to the IGH gene region at 14q32.3.
Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.
Chromosomal translocations involving the IGH locus are recurrent in many types of lymphomas. Burkitt lymphoma (BL) is a rare, but fast growing type of non-Hodgkin lymphoma (NHL). The translocation between the MYC gene locus at 8q24 and the immunoglobulin genes (IG) for the kappa light chain at 2p12 (IGK), for the heavy chain at 14q32 (IGH) or for the lambda light chain at 22q11 (IGL), juxtapose the MYC gene to an IG enhancer, resulting in overexpression of MYC. About 80% of BL patients show the MYC rearrangement t(8;14)(q24;q32) while approximately 10% show a translocation between the MYC gene region and IGK or IGL. Additional breakpoints in BCL2, BCL6 and CCND1 are indicators for an aggressive course and short overall survival.
MYC translocations are also present in other types of lymphomas such as diffuse large B-cell lymphoma (DLBCL), which is difficult to distinguish from BL by morphology and immunophenotype alone. The use of different techniques including FISH, genomic and cytogenetic profiling can provide additional information.
Clinical Applications
- Non-Hodgkin Lymphomas (NHL)
- Acute Lymphoblastic Leukemia (ALL)
XL t(8;14) MYC/IGH DF 8cen hybridized to lymphocytes. One normal interphase and metaphase are shown.
Normal Cell:
Two blue (2B), two green (2G), and two orange (2O) signals.
Aberrant Cell (typical results):
Two blue (2B), one green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.
Aberrant Cell (typical results):
Three blue (3B), two green (2G), and three orange (3O) signals resulting from a trisomy of the orange/blue labeled chromosome.
Aberrant Cell (typical results):
Two blue (2B), two green (2G), and three orange (3O) signals resulting from a reciprocal translocation between the orange labeled and an unknown chromosome.
- Siebert et al (1998) Blood 91:984-990
- Boerma et al (2009) Leukemia 23:225-234
- Nguyen et al (2017) Genes 8:1-23
Fact Sheet (English)Package Insert (EN)Package Insert (IT)Probes Catalog
Certificate of Analysis (CoA)
or go to CoA DatabaseSecond MetaSystems User Day in Altlussheim, Germany
Neon, the outstanding MetaSystems case and image management system, offers many tools and helpful gadgets to streamline routine workflows, for example in cytogenetics labs. The second MetaSystems User Day, addressed to MetaSystems clients from Germany, Austria, and Switzerland, provided in different workshops knowledge helping to unleash the full power of the system.
OOO MetaSystems Russia on the International Scientific Congress 'Genetics of XXI Century'
The Congress 'Genetics of XXI Century' in Moscow, Russia (May 2019) has been one of the most important events for the professional international community of geneticists. Our partner company in Russia OOO MetaSystems considered it a good occasion to present the new camera CoolCube 4 connected to a state-of-the-art Neon system to the public.
