About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
MetaSystems DM2019 Largest in History
XL MYCN amp
Amplification Probe
- Order Number
- D-6031-100-OG
- Package Size
- 100 µl (10 Tests)
- Chromosome
- 022
- Regulatory Status
- IVDD
IVDR Certification
MetaSystems Probes has already certified a large part of its portfolio, according to IVDR. For organizational reasons, we currently provide only the IVDD product.
Discover all IVDR-certified products
XL MYCN amp consists of a green-labeled probe hybridizing to the MYCN gene region at 2p24 and an orange-labeled probe hybridizing to the NMI gene region at 2q23.
Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.
The MYCN gene is a member of the MYC transcription factor family consisting of c-MYC, MYCN and MYCL. It is crucial for embryonic development, especially in the nervous system. The expression is tightly regulated in a spatial and timely manner and high levels are found in the developing brain, retina, neuroepithelial cells, lung and kidney. MYCN expression is associated with the maintenance of self-renewal capacity and the pluripotent status of stem cells. Dysregulation of MYCN contributes to the development of different kinds of childhood tumors including neuroblastoma, medulloblastoma, rhabdomyosarcoma and Wilms tumor. MYCN is also involved in the development of some adulthood neoplasms such as prostate and lung cancer.
Neuroblastoma is the most common extracranial solid tumor in infants. About 6% of all cancers in children are caused by neuroblastomas and 20-25% of neuroblastoma patients are showing an amplification of the MYCN gene. MYCN amplification is an important prognostic marker for risk stratification in neuroblastoma. Generally, patients with MYCN amplification have a poor prognosis. FISH is a valuable tool for the analysis of the MYCN amplification status. It detects MYCN amplification on single-cell level and allows the correlation with morphological cell features.
Clinical Applications
- Solid Tumors (Solid Tumors)
XL MYCN amp hybridized to neuroblastoma tissue. Amplification of MYCN is indicated by multiple copies of the green signal.
Normal Cell:
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two orange (2O), one green (1G) and green signal clusters resulting from an amplification of the green signal (homogenously staining region).
Aberrant Cell (typical results):
Two orange (2O) and multiple green (mG) signals resulting from amplification of the green signal (double minutes).
- Theissen et al (2009) Clin Cancer Res 15:2085-2090
- Huang and Weiss (2013) Cold Spring Harb Perspect Med 2013;3(10):a014415
- Ruiz-Pérez et al (2017) Genes 8(4):113
Fact Sheet (English)Package Insert (EN)Package Insert (IT)Probes Catalog
Certificate of Analysis (CoA)
or go to CoA DatabaseSecond MetaSystems User Day in Altlussheim, Germany
Neon, the outstanding MetaSystems case and image management system, offers many tools and helpful gadgets to streamline routine workflows, for example in cytogenetics labs. The second MetaSystems User Day, addressed to MetaSystems clients from Germany, Austria, and Switzerland, provided in different workshops knowledge helping to unleash the full power of the system.
OOO MetaSystems Russia on the International Scientific Congress 'Genetics of XXI Century'
The Congress 'Genetics of XXI Century' in Moscow, Russia (May 2019) has been one of the most important events for the professional international community of geneticists. Our partner company in Russia OOO MetaSystems considered it a good occasion to present the new camera CoolCube 4 connected to a state-of-the-art Neon system to the public.
